Application
Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using H4R3me2s antibody.Application
Dot-blot analysis of all sorts of methylation peptides using H4R3me2s antibody.Application
Immunofluorescence analysis of 293T cell using H4R3me2s antibody. Blue: DAPI for nuclear staining.| Product Name | Histone H4R3me2 (Symmetric dimethyl Arg3) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H4R3me2 (Symmetric dimethyl Arg3)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide of human Symmetric DiMethyl-Histone H4-R3 |
| Modification | Symmetric dimethyl Arg3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotICC/IFIHC-PWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRatOther |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | histone cluster 2 H4a HIST2H4A H4/n H4FN HIST2H4 H4F2 FO108 H4 |
|---|---|
| Target | Histone H4R3me2 (Symmetric dimethyl Arg3) |
Application
Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using H4R3me2s antibody.
Application
Dot-blot analysis of all sorts of methylation peptides using H4R3me2s antibody.
Application
Immunofluorescence analysis of 293T cell using H4R3me2s antibody. Blue: DAPI for nuclear staining.| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
