Application
Dot Blot analysis of recombinant HIV protease. The total amount of recombinant HIV-protease spotted on the nitrocellulose membrane are indicated in left column. Lane 1: anti-HIV protease (1696) ; 0.2 μg/ml Lane 2: anti-HIV protease (1696) ; 1.0 μg/ml Lane 3: anti-HIV protease (1696) ; 2.0 μg/ml| Product Name | HIV protease antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [1696] to HIV protease |
| Immunogen | Recombinant HIV-1 protease. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1696 |
| Host Species | Mouse |
| Tested Applications | DotELISAInhibitionWB |
| For ELISA: Use at an assay dependent dilution. For Inhib: Use at an assay dependent dilution. Inhibits the enzyme activity of HIV-1 and HIV-2 proteases. For WB (reducing conditions): Use at a concentration of 0 . 5 μ g / m l. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | HIV protease |
|---|---|
| Tissue Specificity | Recognizes free N-terminus of mature HIV protease (HIV-1 and HIV-2), an enzyme that hydrolyzes viral polyproteins into functional proteins |
Application
Dot Blot analysis of recombinant HIV protease. The total amount of recombinant HIV-protease spotted on the nitrocellulose membrane are indicated in left column. Lane 1: anti-HIV protease (1696) ; 0.2 μg/ml Lane 2: anti-HIV protease (1696) ; 1.0 μg/ml Lane 3: anti-HIV protease (1696) ; 2.0 μg/ml| Application Notes | For ELISA: Use at an assay dependent dilution. For Inhib: Use at an assay dependent dilution. Inhibits the enzyme activity of HIV-1 and HIV-2 proteases. For WB (reducing conditions): Use at a concentration of 0 . 5 μ g / m l. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 15mM sodium azide |
