Application
FACS analysis of human peripheal blood lymphocytes using HLA-B7 antibody [BB7.1].| Product Name | HLA-B7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [BB7.1] to HLA-B7HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008] |
| Immunogen | Papain solubilized HLA-B7 antigen |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | BB7.1 |
| Host Species | Mouse |
| Tested Applications | FACS |
| Flow Cytometry: Max Dilution 1/50 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | HumanCynomolgus Monkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HLA-B AS SPDA1 HLAB |
|---|---|
| Target | HLA-DRB7 |
| Tissue Specificity | This antibody recognizes the HLA-B7 antigen and does not cross-react with HLA-B27 or other related antigens. |
Application
FACS analysis of human peripheal blood lymphocytes using HLA-B7 antibody [BB7.1].| Application Notes | Flow Cytometry: Max Dilution 1/50 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide |
