Application
Western Blot analysis of MHC Class II in whole cell lysate of RAJI human Burkitt lymphoma cell line using anti-human HLA-DR+DP (MEM-136) . The antibody MEM-136 recognizes β-chain of HLA-DR antigen under non-reducing conditions. Lane 1: immunostaining with anti-human HLA-DR+DP (MEM-136) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06)| Product Name | HLA-DR + HLA-DP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - 136] to HLA DR + HLA DP |
| Immunogen | PHA-activated peripheral blood lymphocytes. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-136 |
| Host Species | Mouse |
| Tested Applications | FACSIPWB |
| FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | HLA-DR + HLA-DP |
|---|---|
| Tissue Specificity | Common epitope on b-chain of human HLA-DR and HLA-DP. Reacts with alpha-beta dimer as well as with dissociated beta subunit. |
Application
Western Blot analysis of MHC Class II in whole cell lysate of RAJI human Burkitt lymphoma cell line using anti-human HLA-DR+DP (MEM-136) . The antibody MEM-136 recognizes β-chain of HLA-DR antigen under non-reducing conditions. Lane 1: immunostaining with anti-human HLA-DR+DP (MEM-136) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06)| Application Notes | FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
