Application
Western blot analysis of HLA-DR1 in Raji (A) and Jurkat (B) cell lines using MEM-267 antibody .| Product Name | HLA-DR1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Antibody to HLA-DR1 (Human) Purified |
| Immunogen | purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | MEM-267 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSWB |
| FACS: The antibody stains immature dendritic cells that express empty cell surface MHC molecules, but not cells that predominantly peptide loaded forms.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HLA DR1 HLADR1 |
|---|---|
| Target | HLA-DR1 |
| Tissue Specificity | HLA-DR1 belongs to the HLA class II beta chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC). The beta chain is approximately 26-28 kDa. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.The antibody MEM-267 specifically binds to the empty but not peptide-loaded form of HLA-DR1. |
Application
Western blot analysis of HLA-DR1 in Raji (A) and Jurkat (B) cell lines using MEM-267 antibody .| Application Notes | FACS: The antibody stains immature dendritic cells that express empty cell surface MHC molecules, but not cells that predominantly peptide loaded forms.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
