Application
Immunohistochemistry staining of human spleen (paraffin sections) with anti-HLA-E| Product Name | HLA-E antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM-E/06] to HLA EHLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. |
| Immunogen | Bacterially expressed recombinant full length protein refolded with beta2-microglobulin and peptide (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-E/06 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-PIP |
| For FACS: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | HLA E HLAE DKFZp686P19218 EA1.2 EA2.1 HLA-6.2 MHC QA1 HLA 6.2 HLA6.2 |
|---|---|
| Target | HLA-E |
| Tissue Specificity | The antibody recognizes native surface-expressed HLA E, but not denaturated heavy chain of HLA E. The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA A3, A11, B7). However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA A24, A32, B8, B15, B27, B35, B44, B54, C3, C4, C5 and C7. |
| Cellular Localization | Type I membrane protein. |
Application
Immunohistochemistry staining of human spleen (paraffin sections) with anti-HLA-E| Application Notes | For FACS: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Tris buffered saline containing 15 mM sodium azide |
