Application
Immunohistochemistry staining of human spleen (paraffin sections) with anti-HLA-E| Product Name | HLA-E antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - E / 07] to HLA EHLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. |
| Immunogen | Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-E/07 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-PIP |
| FACS: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | HLA E HLAE |
|---|---|
| Target | HLA-E |
| Tissue Specificity | The antibody recognized native surface-expressed HLA E, but not denaturated heavy chain of HLA E. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules: HLA B7 (strongly), HLA B8 (moderately), HLA B27, HLA-B44 (weakly). |
| Cellular Localization | Type I membrane protein. |
Application
Immunohistochemistry staining of human spleen (paraffin sections) with anti-HLA-E| Application Notes | FACS: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
