Application
Western blot analysis of HLA-E Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). HLA-E (arrow) was detected using the purified Pab.Application
Western blot analysis of HLA-E (arrow) using rabbit polyclonal HLA-E Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-E gene.| Product Name | HLA-E antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to HLA-E (Internal)HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 114~144 amino acids from the Center region of human HLA-E. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | name: HLAE name: HLA-E MHC class I antigen E synonyms: HLA-6.2 HLA class I histocompatibility antigen alpha chain E synonyms: HLA6.2 HLAE HLA E name: HLA E synonyms: HLA 6.2 |
|---|---|
| Target | HLA-E |
| Molecular Weight(MW) | 40.157 kDa (note) |
Application
Western blot analysis of HLA-E Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). HLA-E (arrow) was detected using the purified Pab.
Application
Western blot analysis of HLA-E (arrow) using rabbit polyclonal HLA-E Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-E gene.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
