Application
Western blot of Affinity Purified anti-HMNG antibody shows detection of phosphorylated HMGN1 and HMGN2. Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues. Lanes contain: 1. HMGN1, non-phosphorylated 2. HMGN1, phosphorylated 3. HMGN1 delta20E, delta24E, non-phosphorylated 4. HMGN1, delta20E, delta24E, phosphorylated 5. HMGN2, non-phosphorylated 6. HMGN2, phosphorylated Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1000 for this experiment. The blot was procebetaed using a 5 sec exposure time.Application
Human Skin (formalin-fixed, paraffin-embedded) stained with HMGN1 antibody at 20 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.| Product Name | HMG14 (phospho Ser20/Ser24) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HMGN1 (phospho Ser20/Ser24)Chromosomal protein HMG14 and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. The 2 proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function.[supplied by OMIM] |
| Immunogen | Synthetic peptide |
| Modification | p-Ser20/Ser24 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| WB (1:500 - 1:2000), ELISA (1:5000 - 1:25000), IF, IHC-P (20 μg/ml): |
|
| Species Reactivity | HumanMouseChickenDogPigRatOrangutanBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ27265 FLJ31471 HMG14 MGC104230 MGC117425 HMGN1 (phospho S20/S24) HMGN1 (phospho Ser20/Ser24) HMGN1 (pS20/pS24) HMGN1 (pSer20/pSer24) HMGN1 phospho S20/S24 HMGN1 phospho Ser20/Ser24 Phospho HMGN1 (S20/S24) Phospho HMGN1 (Ser20/Ser24) Phospho |
|---|---|
| Target | HMGN1 |
| Tissue Specificity | Amino acids 19-28 of human HMGN protein containing pSer20 and pSer24 |
Application
Western blot of Affinity Purified anti-HMNG antibody shows detection of phosphorylated HMGN1 and HMGN2. Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues. Lanes contain: 1. HMGN1, non-phosphorylated 2. HMGN1, phosphorylated 3. HMGN1 delta20E, delta24E, non-phosphorylated 4. HMGN1, delta20E, delta24E, phosphorylated 5. HMGN2, non-phosphorylated 6. HMGN2, phosphorylated Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1000 for this experiment. The blot was procebetaed using a 5 sec exposure time.
Application
Human Skin (formalin-fixed, paraffin-embedded) stained with HMGN1 antibody at 20 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.| Application Notes | WB (1:500 - 1:2000), ELISA (1:5000 - 1:25000), IF, IHC-P (20 μg/ml): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Storage Buffer | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. |
