Application
HMGA2 antibody [GT763] detects HMGA2 protein at on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. HMGA2 antibody [GT763] dilution: 1:200.Application
HMGA2 antibody [GT763] detects HMGA2 protein at on SkHep1 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SkHep1 xenograft. HMGA2 antibody [GT763] dilution: 1:200.Application
HMGA2 antibody [GT763] detects HMGA2 protein on C2C12 by immunohistochemical analysis. Sample: Paraffin-embedded C2C12. HMGA2 antibody [GT763] dilution: 1:200.Application
HMGA2 antibody [GT763] detects HMGA2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGA2 protein stained by HMGA2 antibody [GT763] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Application
Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 μg of HMGA2 antibody [GT763] . Western blot analysis was performed using HMGA2 antibody [GT763] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [GT763] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
HMGA2 antibody [GT763] detects HMGA2 protein by western blot analysis. A. 30 µg HepG2 whole cell lysate/extract 15% SDS-PAGE HMGA2 antibody [GT763] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | HMGA2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT763] to HMGA2 (high mobility group AT-hook 2)This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HMGA2. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT763 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, ChickenZebrafish (80%), Chicken (82%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Molecular Weight(MW) | 12 kDa (note) (2) |
|---|---|
| Cellular Localization | Nucleus |
Application
HMGA2 antibody [GT763] detects HMGA2 protein at on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. HMGA2 antibody [GT763] dilution: 1:200.
Application
HMGA2 antibody [GT763] detects HMGA2 protein at on SkHep1 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SkHep1 xenograft. HMGA2 antibody [GT763] dilution: 1:200.
Application
HMGA2 antibody [GT763] detects HMGA2 protein on C2C12 by immunohistochemical analysis. Sample: Paraffin-embedded C2C12. HMGA2 antibody [GT763] dilution: 1:200.
Application
HMGA2 antibody [GT763] detects HMGA2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGA2 protein stained by HMGA2 antibody [GT763] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Application
Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 μg of HMGA2 antibody [GT763] . Western blot analysis was performed using HMGA2 antibody [GT763] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [GT763] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
HMGA2 antibody [GT763] detects HMGA2 protein by western blot analysis. A. 30 µg HepG2 whole cell lysate/extract 15% SDS-PAGE HMGA2 antibody [GT763] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | NIH-3T3 , HepG2 , Rat-2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
