Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody diluted at 1:5000.Application
Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody diluted at 1:500.Application
Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using HMGB1 antibody at 1:500 dilution.Application
HMGB1 antibody detects HMGB1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 12 % SDS-PAGE HMGB1 antibody dilution: 1:1000Application
HMGB1 antibody detects HMGB1 protein at nucleus on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. HMGB1 antibody dilution: 1:500.Application
HMGB1 antibody detects HMGB1 protein at nucleus on mouse vein by immunohistochemical analysis. Sample: Paraffin-embedded mouse vein. HMGB1 antibody dilution: 1:500.Application
HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1 protein stained by HMGB1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Immunoprecipitation of HMGB1 protein from 293T whole cell extracts using 5 μg of HMGB1 antibody . Western blot analysis was performed using HMGB1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | HMGB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HMGB1 (high mobility group protein B1)DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, Pig, Rabbit, Chicken, Rhesus Monkey, BovineXenopus laevis (100%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686A04236 antibody HMG1 antibody HMG3 antibody SBP-1 antibody HMGB1 antibody HMG-1 antibody Amphoterin antibody high-mobility group box 1 antibody high mobility group protein B1 antibody high mobility group protein 1 antibody Sulfoglucuronyl |
|---|---|
| Molecular Weight(MW) | 25 kDa (note) |
| Cellular Localization | Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody diluted at 1:5000.
Application
Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody diluted at 1:500.
Application
Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using HMGB1 antibody at 1:500 dilution.
Application
HMGB1 antibody detects HMGB1 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 12 % SDS-PAGE HMGB1 antibody dilution: 1:1000
Application
HMGB1 antibody detects HMGB1 protein at nucleus on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. HMGB1 antibody dilution: 1:500.
Application
HMGB1 antibody detects HMGB1 protein at nucleus on mouse vein by immunohistochemical analysis. Sample: Paraffin-embedded mouse vein. HMGB1 antibody dilution: 1:500.
Application
HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1 protein stained by HMGB1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Immunoprecipitation of HMGB1 protein from 293T whole cell extracts using 5 μg of HMGB1 antibody . Western blot analysis was performed using HMGB1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | 293T , A431 , HeLa , HepG2 , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
