Application
HMGB1 antibody [GT412] detects HMGB1 protein at nucleus in human ovarian cancer by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. HMGB1 antibody [GT412] diluted at 1:250.Application
HMGB1 antibody [GT412] detects HMGB1 protein by western blot analysis. Mouse tissue extracts and Rat tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT412] diluted at 1:1000.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT412] diluted at 1:3000.Application
HMGB1 antibody [GT412] detects HMGB1 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 12 % SDS-PAGE HMGB1 antibody [GT412] dilution: 1:1000| Product Name | HMGB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT412] to HMGB1 (high-mobility group box 1)DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT412 |
| Host Species | Mouse |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, Pig, Rabbit, Chicken, Rhesus Monkey, BovineXenopus laevis (100%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686A04236 antibody HMG1 antibody HMG3 antibody SBP-1 antibody HMGB1 antibody HMG-1 antibody Amphoterin antibody high-mobility group box 1 antibody high mobility group protein B1 antibody high mobility group protein 1 antibody Sulfoglucuronyl |
|---|---|
| Molecular Weight(MW) | 25 kDa (note) |
| Cellular Localization | Nucleus |
Application
HMGB1 antibody [GT412] detects HMGB1 protein at nucleus in human ovarian cancer by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. HMGB1 antibody [GT412] diluted at 1:250.
Application
HMGB1 antibody [GT412] detects HMGB1 protein by western blot analysis. Mouse tissue extracts and Rat tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT412] diluted at 1:1000.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT412] diluted at 1:3000.
Application
HMGB1 antibody [GT412] detects HMGB1 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 12 % SDS-PAGE HMGB1 antibody [GT412] dilution: 1:1000| Positive Control | 293T , A431 , HeLa , HepG2 , Mouse brain , Rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
