Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] diluted at 1:2000.Application
HMGB1 antibody [GT427] detects HMGB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1 protein stained by HMGB1 antibody [GT427] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.Application
HMGB1 antibody [GT427] detects 0 protein by Western blot analysis. A. 30 µg SK-N-SH whole cell lysate/extract B. 30 µg IMR32 whole cell lysate/extract C. 30 µg SK-N-AS whole cell lysate/extract 12 % SDS-PAGE HMGB1 antibody [GT427] dilution: 1:1000Application
HMGB1 antibody [GT427] detects HMGB1 protein by western blot analysis. Mouse tissue extracts and Rat tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] diluted at 1:1000.| Product Name | HMGB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to HMGB1 (high-mobility group box 1)DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT427 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, Pig, Rabbit, Chicken, Rhesus Monkey, BovineXenopus laevis (100%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Molecular Weight(MW) | 25 kDa (note) |
|---|---|
| Cellular Localization | Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] diluted at 1:2000.
Application
HMGB1 antibody [GT427] detects HMGB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1 protein stained by HMGB1 antibody [GT427] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.
Application
HMGB1 antibody [GT427] detects 0 protein by Western blot analysis. A. 30 µg SK-N-SH whole cell lysate/extract B. 30 µg IMR32 whole cell lysate/extract C. 30 µg SK-N-AS whole cell lysate/extract 12 % SDS-PAGE HMGB1 antibody [GT427] dilution: 1:1000
Application
HMGB1 antibody [GT427] detects HMGB1 protein by western blot analysis. Mouse tissue extracts and Rat tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] diluted at 1:1000.| Positive Control | 293T , SK-N-SH , IMR32 , SK-N-AS , Mouse brain , Rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
