Application
Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-HMG-1 Clone: HAP46.5 . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. Mouse kidney 2. HeLa 3. HeLa nuclear 4. Jurkat 5. HEK-293T 6. MDCK 7. PC12 8. NIH-3T3 9. P19| Product Name | HMGB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [HAP46.5] to HMGB1HMG1 and HMG2, chromatin non-histone high mobility group proteins 1 and 2, are 27 and 25 kDa members of a family of proteins containing multiple HMG-boxes, conserved domains of 80 amino acids which mediate DNA binding of many proteins. HMG box domains recognize DNA structure, such as prebent, supercoiled or four way junction DNA, and non- specific DNA sequences. Both HMG1 and HMG2 contain an N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of these proteins contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMG1/2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites, such as steroid hormone receptors, p53, and Oct. HMG1/2 may serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription and replication. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | HAP46.5 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 - 1 μg/ml. Detects a band of approximately 25 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseChickenDogRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | DKFZp686A04236 HMG1 HMG3 SBP-1 SBP 1 SBP1 |
|---|---|
| Target | HMGB1 |
| Cellular Localization | Nuclear |
Application
Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-HMG-1 Clone: HAP46.5 . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. Mouse kidney 2. HeLa 3. HeLa nuclear 4. Jurkat 5. HEK-293T 6. MDCK 7. PC12 8. NIH-3T3 9. P19| Positive Control | Total cell extract of human MCF breast carcinoma cells |
|---|---|
| Application Notes | ELISA: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 - 1 μg/ml. Detects a band of approximately 25 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
