Application
HMGCL antibody detects HMGCL protein by Western blot analysis. A. 50 µg mouse liver lysate/extract 10 % SDS-PAGE HMGCL antibody dilution: 1:10000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HL antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:3000Application
HMGCL antibody detects HMGCL protein by Western blot analysis. A. 50 µg rat liver lysate/extract 10 % SDS-PAGE HMGCL antibody dilution: 1:10000Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HMGCL antibody at 1:500 dilution.| Product Name | HMGCL antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HMGCL (3-hydroxymethyl-3-methylglutaryl-CoA lyase)The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Full length human HMGCL Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (88%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HL antibody HMGCL antibody mitochondrial 3-hydroxy-3-methylglutaryl-CoA lyase antibody HMG-CoA lyase antibody 3-hydroxy-3-methylglutaryl-CoA lyase antibody hydroxymethylglutaryl-CoA lyase mitochondrial antibody hydroxymethylglutaricaciduria antibody |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) |
| Cellular Localization | Mitochondrion matrix |
Application
HMGCL antibody detects HMGCL protein by Western blot analysis. A. 50 µg mouse liver lysate/extract 10 % SDS-PAGE HMGCL antibody dilution: 1:10000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HL antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:3000
Application
HMGCL antibody detects HMGCL protein by Western blot analysis. A. 50 µg rat liver lysate/extract 10 % SDS-PAGE HMGCL antibody dilution: 1:10000
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HMGCL antibody at 1:500 dilution.| Positive Control | H1299 , mouse liver , rat liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
