Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody diluted at 1:2000.Application
Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE diluted at 1:5000Application
hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP A2B1 protein stained by hnRNP A2B1 antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using hnRNP A2B1 antibody at 1:250 dilution.| Product Name | hnRNP A2B1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to hnRNP A2B1 (heterogeneous nuclear ribonucleoprotein A2/B1)This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human hnRNP A2B1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp779B0244 antibody FLJ22720 antibody HNRNPA2 antibody HNRNPB1 antibody HNRPA2 antibody HNRPA2B1 antibody HNRPB1 antibody RNPA2 antibody SNRPB1 antibody HNRNPA2B1 antibody hnRNP A2 / hnRNP B1 antibody heterogeneous nuclear ribonucleoproteins |
|---|---|
| Molecular Weight(MW) | 37 kDa (note) |
| Cellular Localization | Nucleus , nucleoplasm , Cytoplasm (By similarity) |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody diluted at 1:2000.
Application
Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE diluted at 1:5000
Application
hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP A2B1 protein stained by hnRNP A2B1 antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using hnRNP A2B1 antibody at 1:250 dilution.| Positive Control | 293T , A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
