Application
hnRNP A2B1 antibody detects HNRNPA1B1 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 12% SDS-PAGE hnRNP A2B1 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
hnRNP A2B1 antibody detects HNRNPA1B1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12% SDS-PAGE hnRNP A2B1 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. hnRNP A2B1 antibody diluted at 1:500.Application
hnRNP A2B1 antibody detects hnRNP A2B1 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. hnRNP A2B1 antibody dilution: 1:500.Application
hnRNP A2B1 antibody detects hnRNP A2B1 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. hnRNP A2B1 antibody diluted at 1:500.Application
hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP A2B1 protein stained by hnRNP A2B1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.Application
hnRNP A2B1 antibody detects HNRNPA1B1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 12% SDS-PAGE hnRNP A2B1 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
hnRNPA2B1 antibody immunoprecipitates hnRNPA2B1 protein in IP experiments. IP Sample: 4500 µg A549 whole cell lysate/extract A. Immunoprecipitation of hnRNPA2B1 protein by 2 μg of hnRNPA2B1 antibody B. Control with 2 μg of preimmune rabbit IgG The immunoprecipitated hnRNPA2B1 protein was detected by hnRNPA2B1 antibody diluted at 1:2000.| Product Name | hnRNP A2B1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to hnRNP A2B1 (heterogeneous nuclear ribonucleoprotein A2/B1)This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human hnRNP A2B1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp779B0244 antibody FLJ22720 antibody HNRNPA2 antibody HNRNPB1 antibody HNRPA2 antibody HNRPA2B1 antibody HNRPB1 antibody RNPA2 antibody SNRPB1 antibody HNRNPA2B1 antibody hnRNP A2 / hnRNP B1 antibody heterogeneous nuclear ribonucleoproteins |
|---|---|
| Molecular Weight(MW) | 37 kDa (note) (2) |
| Tissue Specificity | Knockdown/Knockout validation was supported by references (PMID:26991015) |
| Cellular Localization | Nucleus , nucleoplasm , Cytoplasm (By similarity) |
Application
hnRNP A2B1 antibody detects HNRNPA1B1 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 12% SDS-PAGE hnRNP A2B1 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
hnRNP A2B1 antibody detects HNRNPA1B1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12% SDS-PAGE hnRNP A2B1 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. hnRNP A2B1 antibody diluted at 1:500.
Application
hnRNP A2B1 antibody detects hnRNP A2B1 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. hnRNP A2B1 antibody dilution: 1:500.
Application
hnRNP A2B1 antibody detects hnRNP A2B1 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. hnRNP A2B1 antibody diluted at 1:500.
Application
hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP A2B1 protein stained by hnRNP A2B1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.
Application
hnRNP A2B1 antibody detects HNRNPA1B1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract E. 30 µg A375 whole cell lysate/extract 12% SDS-PAGE hnRNP A2B1 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
hnRNPA2B1 antibody immunoprecipitates hnRNPA2B1 protein in IP experiments. IP Sample: 4500 µg A549 whole cell lysate/extract A. Immunoprecipitation of hnRNPA2B1 protein by 2 μg of hnRNPA2B1 antibody B. Control with 2 μg of preimmune rabbit IgG The immunoprecipitated hnRNPA2B1 protein was detected by hnRNPA2B1 antibody diluted at 1:2000.| Positive Control | 293T , A431 , HeLa , HepG2 , A375 , Neuro2A , GL261 , C8030 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
