Application
Immunofluorescence analysis of HepG2 cells, using hnRNP Q Antibody. The picture on the right is treated with the synthesized peptide.Application
Western blot analysis of extracts from Jurkat/HuvEc/293 cells, using hnRNP Q Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | hnRNP Q antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to hnRNP QHeterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human hnRNP Q. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| IF: 1:100~1:500 : |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GRYRBP SYNCRI hnRNPQ hnRNP Q cytoplasmic RNA-interacting protein Glycine and tyrosinerich RNAbinding protein Glycine- and tyrosine-rich RNA-binding protein hnRNP-Q GRY-RBP NSAP1 cytoplasmic RNAinteracting protein Synaptotagminbinding GRY RBP |
|---|---|
| Target | hnRNP Q |
| Molecular Weight(MW) | 69 kDa (note) |
| Tissue Specificity | hnRNP Q Antibody detects endogenous levels of total hnRNP Q protein. |
| Cellular Localization | The tyrosine phosphorylated form bound to RNA is found in microsomes (By similarity). Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Expressed predominantly in the nucleoplasm. Expressed predominantly in the nucleoplasm. Expressed p |
Application
Immunofluorescence analysis of HepG2 cells, using hnRNP Q Antibody. The picture on the right is treated with the synthesized peptide.
Application
Western blot analysis of extracts from Jurkat/HuvEc/293 cells, using hnRNP Q Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | IF: 1:100~1:500 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
