Application
Flow cytometric Analysis of Hela cells, using anti-HNRNPF antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HNRNPF mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HNRNPF mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-HNRNPF mouse monoclonal antibody.Application
Flow cytometric Analysis of Jurkat cells, using anti-HNRNPF antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
Anti-HNRNPF mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HNRNPFApplication
Immunofluorescent staining of HepG2 cells using anti-HNRNPF mouse monoclonal antibody .Application
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-HNRNPF mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-HNRNPF mouse monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNRNPF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNRNPF.| Product Name | HNRNPF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5F5] to HNRNPFComponent of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state. |
| Immunogen | Full length human recombinant protein of human HNRNPF (NP_004957) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5F5 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| IHC 1:50 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HNRNPF mcs941 mcs94-1 MGC110997 |
|---|---|
| Target | HNRNPF |
| Tissue Specificity | is specific for human HNRNPF (NP_004957). |
Application
Flow cytometric Analysis of Hela cells, using anti-HNRNPF antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HNRNPF mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HNRNPF mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-HNRNPF mouse monoclonal antibody.
Application
Flow cytometric Analysis of Jurkat cells, using anti-HNRNPF antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
Anti-HNRNPF mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HNRNPF
Application
Immunofluorescent staining of HepG2 cells using anti-HNRNPF mouse monoclonal antibody .
Application
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-HNRNPF mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-HNRNPF mouse monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNRNPF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNRNPF.| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
