Application
WB analysis of indicated lysates using HP1 alpha antibody at a dilution of 1:1,000.Application
ICC/IF analysis of HeLa cells using HP1 alpha antibody at a dilution of 1:300.| Product Name | HP1 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [3G2-H10-A6] to HP1 alphaThis gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
| Immunogen | Purified recombinant human CBX5 protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 3G2-H10-A6 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | chromobox homolog 5 CBX5 HP1A HP1 |
|---|---|
| Target | HP1 alpha |
| Molecular Weight(MW) | 26kDa (note) |
| Cellular Localization | Nucleus. Chromosome |
Application
WB analysis of indicated lysates using HP1 alpha antibody at a dilution of 1:1,000.
Application
ICC/IF analysis of HeLa cells using HP1 alpha antibody at a dilution of 1:300.| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol |
