Application
WB analysis of various cell lysates using HP1 gamma antibody [5G10-F7-A12] at a dilution of 1:1000.Application
ICC/IF analysis of HeLa cells using HP1 gamma antibody [5G10-F7-A12] at a dilution of 1:200.Application
IP analysis was performed with HeLa lysates and either control IgG PR HP1 gamma antibody [5G10-F7-A12]. The precipitates were detected by the same antibody.Application
IHC-P analysis of prostate cancer using HP1 gamma antibody [5G10-F7-A12] at a dilution of 1:200. Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).| Product Name | HP1 gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [5G10-F7-A12] to HP1 gammaAt the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.[provided by RefSeq, Mar 2011] |
| Immunogen | Purified recombinant human HP1-gamma protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5G10-F7-A12 |
| Host Species | Mouse |
| Tested Applications | IDICC/IFIHCIHC-PIPWB |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseMonkeyRatHamster |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HP1-GAMMA HECH CBX3 HP1Hs-gamma chromobox homolog 3 |
|---|---|
| Target | HP1 gamma |
Application
WB analysis of various cell lysates using HP1 gamma antibody [5G10-F7-A12] at a dilution of 1:1000.
Application
ICC/IF analysis of HeLa cells using HP1 gamma antibody [5G10-F7-A12] at a dilution of 1:200.
Application
IP analysis was performed with HeLa lysates and either control IgG PR HP1 gamma antibody [5G10-F7-A12]. The precipitates were detected by the same antibody.
Application
IHC-P analysis of prostate cancer using HP1 gamma antibody [5G10-F7-A12] at a dilution of 1:200. Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol. |
