Application
HPRT antibody detects HPRT1 protein by western blot analysis. A. 50 µg mouse liver lysate/extract 12% SDS-PAGE HPRT antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HPRT antibody detects HPRT protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HPRT antibody diluted at 1:500.Application
HPRT antibody detects HPRT protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. HPRT antibody diluted at 1:500.Application
HPRT antibody detects HPRT protein at cytoplasm in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. HPRT antibody diluted at 1:500.Application
HPRT antibody detects HPRT protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. HPRT antibody diluted at 1:500.Application
HPRT antibody detects HPRT protein at cytoplasm in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. HPRT antibody diluted at 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HPRT antibody at 1:200 dilution.Application
Sample (30 µg of whole cell lysate) A: Molt-4 B: Raji 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | HPRT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HPRT (hypoxanthine phosphoribosyltransferase 1)HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate (Keebaugh et al., 2007 [PubMed 16928426]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HPRT. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Dog, Pig, Rabbit, Chicken, Chimpanzee, BovineZebrafish (93%), Xenopus laevis (92%), Dog (96%), Pig (97%), Rabbit (98%), Chicken (94%), Chimpanzee (99%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HGPRT antibody HPRT antibody HPRT1 antibody hypoxanthine-guanine phosphoribosyltransferase antibody HGPRTase antibody hypoxanthine phosphoribosyltransferase 1 antibody |
|---|---|
| Molecular Weight(MW) | 25 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
HPRT antibody detects HPRT1 protein by western blot analysis. A. 50 µg mouse liver lysate/extract 12% SDS-PAGE HPRT antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HPRT antibody detects HPRT protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HPRT antibody diluted at 1:500.
Application
HPRT antibody detects HPRT protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. HPRT antibody diluted at 1:500.
Application
HPRT antibody detects HPRT protein at cytoplasm in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. HPRT antibody diluted at 1:500.
Application
HPRT antibody detects HPRT protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. HPRT antibody diluted at 1:500.
Application
HPRT antibody detects HPRT protein at cytoplasm in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. HPRT antibody diluted at 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HPRT antibody at 1:200 dilution.
Application
Sample (30 µg of whole cell lysate) A: Molt-4 B: Raji 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , Molt-4 , Raji , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
