Application
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:1000.Application
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:500.Application
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:500.Application
Immunofluorescence analysis of methanol-fixed A549, using HPRT antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using HPRT antibody at 1:500 dilution.| Product Name | HPRT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HPRT (hypoxanthine phosphoribosyltransferase 1)HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate (Keebaugh et al., 2007 [PubMed 16928426]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HPRT. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Dog, Pig, Rabbit, Chicken, Chimpanzee, BovineZebrafish (92%), Xenopus laevis (90%), Dog (96%), Pig (97%), Rabbit (98%), Chicken (91%), Chimpanzee (99%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HGPRT antibody HPRT antibody HPRT1 antibody hypoxanthine-guanine phosphoribosyltransferase antibody HGPRTase antibody hypoxanthine phosphoribosyltransferase 1 antibody |
|---|---|
| Molecular Weight(MW) | 25 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:1000.
Application
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:500.
Application
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:500.
Application
Immunofluorescence analysis of methanol-fixed A549, using HPRT antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using HPRT antibody at 1:500 dilution.| Positive Control | 293T , A431 , HeLa , HepG2 , PC-12 , Rat2 , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
