Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSC70 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HSC70 antibody detects HSC70 protein at nucleolus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. HSC70 antibody dilution: 1:200.Application
Immunofluorescence analysis of methanol-fixed HeLa, using HSC70 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using HSC70 antibody at 1:100 dilution.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HSC70 antibody detects HSC70 protein at nucleolus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. HSC70 antibody dilution: 1:500.| Product Name | HSC70 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Hsc70 (heat shock 70kDa protein 8)The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human HSC70. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB:1:500~1:2000 ICC/IF:1:100~1:500 IHC-P:1:50~1:200 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineXenopus laevis (91%), Pig (100%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HSC54 antibody HSC70 antibody HSC71 antibody HSP71 antibody HSP73 antibody HSPA10 antibody LAP1 antibody MGC131511 antibody MGC29929 antibody NIP71 antibody HSPA8 antibody heat shock cognate 71 kDa protein antibody heat shock cognate protein 54 |
|---|---|
| Molecular Weight(MW) | 71 kDa (note) |
| Cellular Localization | Cytoplasm , Melanosome |
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSC70 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HSC70 antibody detects HSC70 protein at nucleolus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. HSC70 antibody dilution: 1:200.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using HSC70 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using HSC70 antibody at 1:100 dilution.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HSC70 antibody detects HSC70 protein at nucleolus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. HSC70 antibody dilution: 1:500.| Positive Control | A549 , H1299 , HCT116 , Mouse brain |
|---|---|
| Application Notes | WB:1:500~1:2000 ICC/IF:1:100~1:500 IHC-P:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
