Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using HSC70 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 C: HeLa D: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (20 µg of whole cell lysate) A: Raw264.7 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | HSC70 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Hsc70 (heat shock 70kDa protein 8)The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HSC70. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMousePig |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, BovineRat (100%), Zebrafish (97%), Xenopus laevis (98%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HSC54 antibody HSC70 antibody HSC71 antibody HSP71 antibody HSP73 antibody HSPA10 antibody LAP1 antibody MGC131511 antibody MGC29929 antibody NIP71 antibody HSPA8 antibody heat shock cognate 71 kDa protein antibody heat shock cognate protein 54 |
|---|---|
| Molecular Weight(MW) | 71 kDa (note) |
| Cellular Localization | Cytoplasm , Melanosome |
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using HSC70 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 C: HeLa D: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (20 µg of whole cell lysate) A: Raw264.7 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , Raw264.7 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
