Application
HSD17B4 antibody detects HSD17B4 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: HSD17B4 protein stained by HSD17B4 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HSD17B4 antibody at 1:500 dilution.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:1000| Product Name | HSD17B4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSD17B4 (hydroxysteroid (17-beta) dehydrogenase 4)The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human HSD17B4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, Chicken, Bovine, Guinea pigRat (92%), Zebrafish (85%), Xenopus laevis (81%), Pig (87%), Chicken (84%), Bovine (87%), Guinea pig (85%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DBP antibody MFE-2 antibody MPF-2 antibody SDR8C1 antibody HSD17B4 antibody short chain dehydrogenase/reductase family 8C member 1 antibody peroxisomal multifunctional protein 2 antibody 17beta-estradiol dehydrogenase type IV antibody D-3-hydroxya |
|---|---|
| Molecular Weight(MW) | 80 kDa (note) (5) |
| Cellular Localization | Peroxisome |
Application
HSD17B4 antibody detects HSD17B4 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: HSD17B4 protein stained by HSD17B4 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HSD17B4 antibody at 1:500 dilution.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:1000| Positive Control | HeLa , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
