Application
Immunofluorescence analysis of methanol-fixed Hep3B, using HSD17B4 antibody at 1:500 dilution.Application
HSD17B4 antibody detects HSD17B4 protein by western blot analysis. A. 20 µg Rat heart lysate/extract 7.5% SDS-PAGE HSD17B4 antibody dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft , using HSD17B4 antibody at 1:500 dilution.Application
HSD17B4 antibody detects HSD17B4 protein at mitochondria in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSD17B4 antibody diluted at 1:500.Application
HSD17B4 antibody detects HSD17B4 protein at mitochondria in rat heart by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. HSD17B4 antibody diluted at 1:500.| Product Name | HSD17B4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSD17B4 (hydroxysteroid (17-beta) dehydrogenase 4)The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HSD17B4. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.: WB:1:500~1:1000 IHC-P:1:50~1:100 ICC/IF:1:100~1:500 |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Pig, Bovine, Guinea pigMouse (87%), Pig (87%), Bovine (88%), Guinea pig (84%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DBP antibody MFE-2 antibody MPF-2 antibody SDR8C1 antibody HSD17B4 antibody |
|---|---|
| Molecular Weight(MW) | 80 kDa (note) (5) |
| Cellular Localization | Peroxisome |
Application
Immunofluorescence analysis of methanol-fixed Hep3B, using HSD17B4 antibody at 1:500 dilution.
Application
HSD17B4 antibody detects HSD17B4 protein by western blot analysis. A. 20 µg Rat heart lysate/extract 7.5% SDS-PAGE HSD17B4 antibody dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft , using HSD17B4 antibody at 1:500 dilution.
Application
HSD17B4 antibody detects HSD17B4 protein at mitochondria in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. HSD17B4 antibody diluted at 1:500.
Application
HSD17B4 antibody detects HSD17B4 protein at mitochondria in rat heart by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. HSD17B4 antibody diluted at 1:500.| Positive Control | Rat heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.: WB:1:500~1:1000 IHC-P:1:50~1:100 ICC/IF:1:100~1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
