Mouse monoclonal antibody [J7F9] to HSF1The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq, Jul 2008]
Immunogen
Recombinant human HSF1 (1-529aa) purified from E. coli
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
J7F9
Host Species
Mouse
Tested Applications
ELISAWB
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000~ 2,000. Recommended starting dilution is 1:1,000.:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
heat shock transcription factor 1 HSTF1
Target
HSF1
Light Chain Type
kappa
Application
Application
WB analysis of HeLa lysate (30ug) using HSF1 antibody at a dilution of 1:1,000.
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000~ 2,000. Recommended starting dilution is 1:1,000.:
Additional Information
Form
Liquid
Storage Instructions
Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Storage Buffer
Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.