Application
Non-transfected (–) and transfected (+) HT-29 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody diluted at 1:5000.Application
HSP27 antibody immunoprecipitates HSP27 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of HSP27 protein by 2.5 μg of HSP27 15% SDS-PAGE The immunoprecipitated HSP27 protein was detected by HSP27 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Wild-type (WT) and HSP27 knownout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 12% SDS PAGE diluted at 1:1000Application
HSP27 antibody detects HSP27 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSP27 protein stained by HSP27 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using HSP27 antibody at 1:500 dilution.| Product Name | HSP27 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSP27 (heat shock 27kDa protein 1)The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Rhesus Monkey, BovineMouse (85%), Rat (85%), Dog (88%), Pig (90%), Rhesus Monkey (100%), Bovine (86%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CMT2F antibody DKFZp586P1322 antibody HMN2B antibody HS.76067 antibody HSP27 antibody HSP28 antibody Hsp25 antibody SRP27 antibody HSPB1 antibody 28 kDa heat shock protein antibody stress-responsive protein 27 antibody heat shock protein beta-1 |
|---|---|
| Molecular Weight(MW) | 23 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus , Spindle |
Application
Non-transfected (–) and transfected (+) HT-29 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody diluted at 1:5000.
Application
HSP27 antibody immunoprecipitates HSP27 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of HSP27 protein by 2.5 μg of HSP27 15% SDS-PAGE The immunoprecipitated HSP27 protein was detected by HSP27 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Wild-type (WT) and HSP27 knownout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 12% SDS PAGE diluted at 1:1000
Application
HSP27 antibody detects HSP27 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSP27 protein stained by HSP27 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using HSP27 antibody at 1:500 dilution.| Positive Control | 293T , A431 , HeLa , HepG2 , HT-29 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
