| Product Name | Hsp70 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [BRM - 22] to Hsp70Heat shock proteins are produced by prokaryotic and eukaryotic cells, and are among the most conserved molecules in phylogeny. Eukaryotic cells contain an HSP70 multigene that differ in their intracellular location and regulation (ref 2,3). Members of the HSP70 family play a major role in the folding, unfolding and translocation of polypeptides as well as in the assembly and disassembly of oligomeric protein complexes (ref 4), as well as in the immune response (ref 5). HSP72 was found to increase dramatically in the brains of Alzheimer's disease patient, and was localized exclusively in neuritic plaques and neurofibrillary tangles (ref 7). HSP70 concentrates in nuclei during heat shock and returns to the cytoplasm when the shock is removed (ref 8). |
| Immunogen | Full length native protein (purified) (Bovine brain). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | BRM-22 |
| Host Species | Mouse |
| Tested Applications | DotEMELISAICC/IFIHC-FWB |
| Dot: Use at a dilution of 1/5000. Electron Microscopy: Use at an assay dependant dilution. IF: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. I-ELISA: Use at an assay dependant dilution. WB: Use at a dilution of 1/5000. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanChickenDrosophilaPigRatC.elegansGuinea PigHamsterRabbitPlantBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Hsp70 |
|---|---|
| Tissue Specificity | Monoclonal Anti-Heat Shock Protein 70 (HSP70) 70 reacts specifically against HSP70 in ELISA and immuno-blotting. In immunoblotting the antibody localizes both the constitutive (HSP73) and inducible (HSP72) forms of HSP70. The antibody recognizes brain HSP70 of bovine, human, rat, rabbit, chicken, and guinea pig. It also recognizes HSP70 in Drosophila cell extract, nematode and plant as well as human fibroblast cell extract. Immunofluorescent staining demonstrates a rapid and reversible accumulation of the HSP70 protein within the nucleus of heat-stressed (42ºC, 1 hr.) human fibroblasts. |
| Cellular Localization | cytoplasmic |
| Application Notes | Dot: Use at a dilution of 1/5000. Electron Microscopy: Use at an assay dependant dilution. IF: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. I-ELISA: Use at an assay dependant dilution. WB: Use at a dilution of 1/5000. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Ascites fluid with 15mM sodium azide |
