Application
Western blot analysis of Hsp90 with heat shocked human HeLa cell lysate| Product Name | Hsp90 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [S88] to Hsp90Hsp90 (also known as Hsp82, Hsp83, or Hsp89), a 90 kDa protein, is the most abundant protein in the cytosolic fraction in many cell types. The high level of Hsp90 in many cell types suggests that Hsp90 may play a general role in the cell, but little is known about its general function. Hsp90 has no known enzymatic activity, and thus it has been presumed that Hsp90 may function through protein-protein interactions. Hsp90 exists in a dimeric form and has been observed to bind to several cellular proteins such as retro-virus kinases (PP60v-src), steroid receptors, heme-regulated protein kinase, actin and tubulin. In this regard, Hsp90, like Hsp70, may function as a molecular chaperone. Hsp90 exists as two isoforms referred to as Hsp86 and HSP84 in murine cells or Hsp90a and Hsp90b in human cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | S88 |
| Host Species | Mouse |
| Tested Applications | IHC-FIPWB |
| IHC-Fr: Use at a concentration of 10 - 20 μg/ml. IP: Use at a concentration of 4 μg/ml. WB: Use at a concentration of 1 μg/ml. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseMonkeyPigRatRabbitSheepBovine |
| Concentration | 0.2mg/ml |
| Gene Synonyms | FLJ31884 heat shock 90kDa protein 1 alpha Heat shock protein HSP 90 alpha HSP86 Hsp89 HSP90A HSPC1 HSPCAL1 HSPN LAP2 Lipopolysaccharide associated protein2 LPS associated protein 2 |
|---|---|
| Target | Hsp90 |
| Tissue Specificity | The antibody recognizes a 90 kDa protein, corresponding to the apparent molecular weight of Hsp90 on SDS-PAGE immunoblots. This antibody detects both Hsp90 alpha and beta. |
| Cellular Localization | Cytoplasmic |
Application
Western blot analysis of Hsp90 with heat shocked human HeLa cell lysate| Application Notes | IHC-Fr: Use at a concentration of 10 - 20 μg/ml. IP: Use at a concentration of 4 μg/ml. WB: Use at a concentration of 1 μg/ml. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide |
