Application
HEK293T cells transfected with either RC207795 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HSPA6 antibody, and then analyzed by flow cytometry.Application
Anti-HSPA6 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HSPA6Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA6 monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA6.| Product Name | HSPA6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1H3] to HSPA6In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. |
| Immunogen | Full length human recombinant protein of human HSPA6 (NP_002146) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 1H3 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | HSPA6 |
|---|---|
| Tissue Specificity | is specific for human HSPA6 (NP_002146). |
Application
HEK293T cells transfected with either RC207795 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HSPA6 antibody, and then analyzed by flow cytometry.
Application
Anti-HSPA6 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HSPA6
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA6 monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA6.| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
