Application
NCI-H929 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HTRA2 antibody diluted at 1:500.| Product Name | HTRA2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HTRA2 (HtrA serine peptidase 2)This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human HTRA2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, ChimpanzeeRhesus Monkey (100%), Chimpanzee (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PRSS25 OMI PARK13 HtrA serine peptidase 2 |
|---|---|
| Target | HTRA2 |
| Molecular Weight(MW) | 49 kDa (note) |
| Cellular Localization | Mitochondrion intermembrane space , Mitochondrion membrane; Single-pass membrane protein (Potential) |
Application
NCI-H929 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HTRA2 antibody diluted at 1:500.| Positive Control | NCI-H929 , NCI-H929 membrane fraction extract |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
