Application
Sandwich ELISA analysis of human IgE using Mouse anti Human IgE as a capture reagent and horseradish peroxidase conjugated Mouse anti Human IgE as a detection reagent with purified human IgE as antigen. A serum sample at 1/10 (blue) and 1/5 (green) and a plasma sample at 1/10 (red) and1/5 (orange) is shown.| Product Name | Human IgE antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [4C3] to Human IgE |
| Immunogen | Chimaeric human IgE |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4C3 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| For ELISA: Use at 5ug/mL as captive antibody: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Target | Human IgE |
|---|---|
| Tissue Specificity | recognises human IgE and shows no cross-reactivity to other human immunoglobulin classes or subclasses. |
Application
Sandwich ELISA analysis of human IgE using Mouse anti Human IgE as a capture reagent and horseradish peroxidase conjugated Mouse anti Human IgE as a detection reagent with purified human IgE as antigen. A serum sample at 1/10 (blue) and 1/5 (green) and a plasma sample at 1/10 (red) and1/5 (orange) is shown.| Application Notes | For ELISA: Use at 5ug/mL as captive antibody: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline, 0.09%Sodium Azide |
