Application
IgG κ light chain (1), IgG λ light chain (2) and IgG Fc fragment (3) purified from human serum were analysed by Western blotting with MEM-09 antibody against IgG κ light chain (A) and EM-07 antibody against IgG Fc fragment (B).| Product Name | Human IgG (Fc) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [EM-07] to Human IgG (Fc)Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins. |
| Immunogen | Fusion protein of human IgG Fc fragment. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | EM-07 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| WB: Note: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments by WB. The antibody EM-07 does not crossreact with IgM: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Target | Human IgG (Fc) |
|---|---|
| Tissue Specificity | The antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments. |
Application
IgG κ light chain (1), IgG λ light chain (2) and IgG Fc fragment (3) purified from human serum were analysed by Western blotting with MEM-09 antibody against IgG κ light chain (A) and EM-07 antibody against IgG Fc fragment (B).| Application Notes | WB: Note: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments by WB. The antibody EM-07 does not crossreact with IgM: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
