Application
Western Blot analysis (reducing conditions) of human IgG Fab fragment using anti-human IgG Fab fragment Lane 1: Normal human immunoglobulins Lane 2: Normal bovine immunoglobulins| Product Name | Human IgG Fab fragment antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [4A11] to Human IgG Fab fragment |
| Immunogen | Purified human IgG |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 4A11 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| For ELISA: Use at an assay dependent diluiton. For WB: Use at an assay dependent diluiton. Optimal dilutions/concentrations should be determined by the researcher: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | Human IgG Fab fragment |
|---|---|
| Tissue Specificity | Fab fragment of human IgG. Specificity was confirmed by Western blotting analysis of purified Fab fragment, of human IgG, under reducing conditions. |
Application
Western Blot analysis (reducing conditions) of human IgG Fab fragment using anti-human IgG Fab fragment Lane 1: Normal human immunoglobulins Lane 2: Normal bovine immunoglobulins| Application Notes | For ELISA: Use at an assay dependent diluiton. For WB: Use at an assay dependent diluiton. Optimal dilutions/concentrations should be determined by the researcher: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 15mM sodium azide |
