| Product Name | Human IgM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [47.2.E9] to IgMImmunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; |
| Immunogen | Purified human IgM |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 47.2.E9 |
| Host Species | Mouse |
| Tested Applications | ELISA |
| ELISA: 1/5000 - 1/10000 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AGM1 IGHM MU immunoglobulin heavy constant mu VH |
|---|---|
| Target | IgM |
| Tissue Specificity | This antibody recognizes the Mu chain. |
| Application Notes | ELISA: 1/5000 - 1/10000 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide (NaN3) |
