Application
Surface staining of human peripheral blood cells with anti-human IgM : APC. Cells in the lymphocyte gate were used for analysis.| Product Name | Human IgM Fc fragment antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [CH2] to Human IgM Fc fragment |
| Immunogen | Full length native protein (purified) (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | CH2 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| ELISA: Use at an assay dependant concentration. FACS: Use at an assay dependant concentration. IF: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. This antibody blocks binding of standard antibody to Fc fragment of human IgM and does not block binding of standard antibody to Fab fragment of human IgM. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | Human IgM Fc fragment |
|---|---|
| Tissue Specificity | This antibody reacts with Fc fragment of human IgM. Specificity was confirmed by Western blotting analysis of purified human IgM, under reducing conditions, and also by immunofluorescence staining of Daudi cells |
Application
Surface staining of human peripheral blood cells with anti-human IgM : APC. Cells in the lymphocyte gate were used for analysis.| Application Notes | ELISA: Use at an assay dependant concentration. FACS: Use at an assay dependant concentration. IF: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. This antibody blocks binding of standard antibody to Fc fragment of human IgM and does not block binding of standard antibody to Fab fragment of human IgM. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not freeze. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
