Application
B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2 antibody, CD19 by LT19 antibody.?| Product Name | Human kappa light chain antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [A8B5] to Human kappa light chainImmunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | A8B5 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIHC-FIHC-P |
| FACS: Positive Control: Daudi human cell line: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | LYT-10 LYT10 |
|---|---|
| Target | Human kappa light chain |
| Tissue Specificity | The antibody A8B5 reacts with kappa light chains (22.5 kDa) of immunoglobulins. |
| Cellular Localization | Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor. |
Application
B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2 antibody, CD19 by LT19 antibody.?| Application Notes | FACS: Positive Control: Daudi human cell line: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
