| Product Name | Human kappa Light chain antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [KP53] to Human kappa Light chain |
| Immunogen | BALB/C mice were immunized with purified Bence Jones kappa light chain protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | KP53 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| This antibody has been pretitered and quality controlled to work allon formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Athough not required, enzymatic predigestion may help in some cases.cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Athough not required, enzymatic predigestion may help in some cases.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Target | Human kappa Light chain |
|---|---|
| Tissue Specificity | This antibody reacts with B cell follicles in human lymphoid tissues. The mantle zones give a mosaic pattern, while the germinal centers show a coarse meshwork pattern of staining. This antibody stains free as well as bound kappa light chains. This antibody does not react with human lambda light chain. |
| Application Notes | This antibody has been pretitered and quality controlled to work allon formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Athough not required, enzymatic predigestion may help in some cases.cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Athough not required, enzymatic predigestion may help in some cases.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS with sodium azide |
