Application
Western Blot analysis (reducing conditions) of human blood sera using anti-human Immunoglobulin kappa-chain . Lane 1-3: Human blood serum of different healthy donors| Product Name | Human kappa Light chain antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [MEM-09] to Human kappa Light chain |
| Immunogen | Tissue / cell preparation (Human). (Crude thymus membrane fraction). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-09 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PIPWB |
| FACS: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC72072 Km MGC62011 MGC111575 HCAK1 MGC88770 kappa Light chain MGC88771 MGC88809 |
|---|---|
| Target | Human kappa Light chain |
| Tissue Specificity | MAb MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with k-light chains. Material immunoprecipitated from human serum using MAb MEM-09 consist of IgG and traces of IgM. |
Application
Western Blot analysis (reducing conditions) of human blood sera using anti-human Immunoglobulin kappa-chain . Lane 1-3: Human blood serum of different healthy donors| Application Notes | FACS: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
