B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2 antibody, CD19 by LT19 antibody.
Mouse Monoclonal antibody [4C2] to Human lambda light chainImmunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4C2
Host Species
Mouse
Tested Applications
ELISAFACSIHC-FIHC-P
FACS: Positive Control: RAMOS human cell line:
Species Reactivity
Human
Concentration
1mg/ml
Target Information
Target
Human lambda light chain
Tissue Specificity
The antibody 4C2 reacts with lambda light chains (22.5 kDa) of human immunoglobulin.
Application
Application
B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2 antibody, CD19 by LT19 antibody.
Application Notes
FACS: Positive Control: RAMOS human cell line:
Additional Information
Form
Liquid
Storage Instructions
Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles.
Storage Buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4