Application
Western Blot: Huntingtin Antibody (1A771) - Western blot analysis of Huntington Disease protein in different lymphoblast cell line extracts (Lanes 1-4) (CAG repeats ~65 and ~20) using anti-huntingtin mAb (Panel A) and an antibody from another source (Panel B).| Product Name | Huntingtin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [1A771] to HuntingtinHuntington's disease (HD) is a neurodegenerative disorder caused by an expanding polyglutamine repeat in the huntingtin gene. HD is a mid-life onset autosomal dominant neurodegeneative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years. The HD gene product is widely expressed in human tissues, with the highest level of expression in the brain. The huntingtin gene product is expressed at similar levels in patients and controls, which suggests that the expansion of the polyglutamine repeat induces a toxic gain of function perhaps through interactions with other cellular proteins. Using yeast two-hybrid system, HAP1 (huntingtin associated protein 1) has been identified, that associates with huntingtin protein. The In vitro data suggest that the association between HAP1 and huntingtin is enhanced by increasing length of glutamine repeat. |
| Immunogen | The antibody was raised against a fusion protein containing 62 glutamine amino acid repeats. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1A771 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 377 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | HD HD protein HDH HTT HUNTINGTON CHOREA Huntington's disease protein homolog IT15 ZHD |
|---|---|
| Target | Huntingtin |
| Cellular Localization | Cytoplasmic |
Application
Western Blot: Huntingtin Antibody (1A771) - Western blot analysis of Huntington Disease protein in different lymphoblast cell line extracts (Lanes 1-4) (CAG repeats ~65 and ~20) using anti-huntingtin mAb (Panel A) and an antibody from another source (Panel B).| Positive Control | Lymphoblast cell line extracts |
|---|---|
| Application Notes | WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 377 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
