Application
Iba1 antibody detects Iba1 protein at cytoplasm on human colon carcinoma stroma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma stroma. Iba1 antibody dilution: 1:500.Application
Iba1 antibody detects Iba1 protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Iba1 protein stained by Iba1 antibody diluted at 1:400. Blue: Hoechst 33342 staining.Application
Iba1 detects Iba1 protein at cytosol on mouse brain tissue by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain tissue. Iba1 dilution: 1:1000.Application
Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Iba1 antibody dilution: 1:500.Application
Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. Iba1 antibody dilution: 1:500.Application
Iba1 antibody detects Iba1 by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: Iba1 antibody dilution: 1:50.Application
Iba1 antibody detects Iba1 protein by western blot analysis. A. 30 µg THP-1 whole cell lysate/extract B. 30 µg HL-60 whole cell lysate/extract 15% SDS-PAGE Iba1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Iba1 antibody detects Iba1 protein expression at microglias by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Iba1 protein stained by Iba1 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: Molt-4 15% SDS PAGE AIF1 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Iba1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Iba1 (allograft inflammatory factor 1)This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-FIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rhesus MonkeyPig (100%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AIF-1 antibody IBA1 antibody IRT-1 antibody IRT1 antibody AIF1 antibody ionized calcium-binding adapter molecule 1 antibody allograft inflammatory factor 1 antibody interferon gamma responsive transcript antibody protein G1 antibody |
|---|---|
| Molecular Weight(MW) | 17 kDa (note) (3) |
Application
Iba1 antibody detects Iba1 protein at cytoplasm on human colon carcinoma stroma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma stroma. Iba1 antibody dilution: 1:500.
Application
Iba1 antibody detects Iba1 protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Iba1 protein stained by Iba1 antibody diluted at 1:400. Blue: Hoechst 33342 staining.
Application
Iba1 detects Iba1 protein at cytosol on mouse brain tissue by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain tissue. Iba1 dilution: 1:1000.
Application
Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Iba1 antibody dilution: 1:500.
Application
Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. Iba1 antibody dilution: 1:500.
Application
Iba1 antibody detects Iba1 by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: Iba1 antibody dilution: 1:50.
Application
Iba1 antibody detects Iba1 protein by western blot analysis. A. 30 µg THP-1 whole cell lysate/extract B. 30 µg HL-60 whole cell lysate/extract 15% SDS-PAGE Iba1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Iba1 antibody detects Iba1 protein expression at microglias by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Iba1 protein stained by Iba1 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: Molt-4 15% SDS PAGE AIF1 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Molt-4 , K562 , THP-1 , HL-60 , mouse liver, rat liver , *Mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
