Application
Iba1 antibody detects Iba1 protein at cytoplasm on human breast cancer stroma by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer stroma. Iba1 antibody dilution: 1:500.Application
Immunoprecipitation of Iba1 protein from K562 whole cell extracts using 5 μg of Iba1 antibody . Western blot analysis was performed using Iba1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Iba1 antibody detects Iba1 by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: Iba1 antibody dilution: 1:50.Application
Iba1 antibody detects Iba1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody diluted at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Iba1 antibody detects Iba1 protein expression at microglias by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Iba1 protein stained by Iba1 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .Application
Iba1 antibody detects Iba1 protein at microglia in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Iba1 antibody diluted at 1:500.Application
Iba1 antibody detects Iba1 protein at microglia in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Iba1 antibody diluted at 1:500.Application
Flow cytometry on primary murine microglia cells, staining with Iba1 antibody using 1.0 µg per 4×105 cells. (blue), Rabbit IgG (green) ,Unstained (red).| Product Name | Iba1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Iba1 (allograft inflammatory factor 1)This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Iba1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHCIHC-FIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rhesus Monkey, BovinePig (93%), Rhesus Monkey (99%), Bovine (88%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AIF-1 antibody IBA1 antibody IRT-1 antibody IRT1 antibody AIF1 antibody ionized calcium-binding adapter molecule 1 antibody allograft inflammatory factor 1 antibody interferon gamma responsive transcript antibody protein G1 antibody |
|---|---|
| Molecular Weight(MW) | 17 kDa (note) |
Application
Iba1 antibody detects Iba1 protein at cytoplasm on human breast cancer stroma by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer stroma. Iba1 antibody dilution: 1:500.
Application
Immunoprecipitation of Iba1 protein from K562 whole cell extracts using 5 μg of Iba1 antibody . Western blot analysis was performed using Iba1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Iba1 antibody detects Iba1 by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: Iba1 antibody dilution: 1:50.
Application
Iba1 antibody detects Iba1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody diluted at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Iba1 antibody detects Iba1 protein expression at microglias by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Iba1 protein stained by Iba1 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
Application
Iba1 antibody detects Iba1 protein at microglia in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Iba1 antibody diluted at 1:500.
Application
Iba1 antibody detects Iba1 protein at microglia in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Iba1 antibody diluted at 1:500.
Application
Flow cytometry on primary murine microglia cells, staining with Iba1 antibody using 1.0 µg per 4×105 cells. (blue), Rabbit IgG (green) ,Unstained (red).| Positive Control | THP-1 , HL-60 , mouse liver, rat liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
