Application
Iba1 antibody [GT10312] detects Iba1 protein at microglia in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Iba1 antibody [GT10312] diluted at 1:200.Application
Iba1 antibody [GT10312] detects Iba1 protein at microglia in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Iba1 antibody [GT10312] diluted at 1:200.Application
Iba1 antibody detects Iba1 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Iba1 antibody [GT10312] detects Iba1 protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Iba1 protein stained by Iba1 antibody [GT10312] diluted at 1:200. Blue: Hoechst 33342 staining.| Product Name | Iba1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT10312] to Iba1 (allograft inflammatory factor 1)This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Iba1. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT10312 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Rhesus Monkey, BovineRat (89%), Pig (93%), Rhesus Monkey (99%), Bovine (88%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | allograft inflammatory factor 1 AIF-1 IRT1 IBA1 AIF1 IRT-1 |
|---|---|
| Target | Iba1 |
| Molecular Weight(MW) | 17 kDa (note) |
Application
Iba1 antibody [GT10312] detects Iba1 protein at microglia in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Iba1 antibody [GT10312] diluted at 1:200.
Application
Iba1 antibody [GT10312] detects Iba1 protein at microglia in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Iba1 antibody [GT10312] diluted at 1:200.
Application
Iba1 antibody detects Iba1 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Iba1 antibody [GT10312] detects Iba1 protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Iba1 protein stained by Iba1 antibody [GT10312] diluted at 1:200. Blue: Hoechst 33342 staining.| Positive Control | THP-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
