Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DFFA monoclonal antibody.Application
Anti-DFFA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with DFFA| Product Name | ICAD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3F12] to ICADInhibitor of the caspase-activated DNase (DFF40). |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 1-331 of human DFF45 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3F12 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| ICC/IF (1:100): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DFFA DFF-45 DFF1 DFF45 ICAD DFF 45 |
|---|---|
| Target | ICAD |
| Tissue Specificity | is specific for amino acids 1-331 of human DFF45 |
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DFFA monoclonal antibody.
Application
Anti-DFFA mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with DFFA| Application Notes | ICC/IF (1:100): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
