Application
Western blottin analysis of CD54 expression in TNF-alpha activated (A) and nonactivated (B) HUVEC cells by Lower bands represent tubulin as a loading control.| Product Name | ICAM1 / CD54 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - 111] to ICAM1Human CD54, also known as intercellular adhesion molecule-1 (ICAM1), is a 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with CD50 (ICAM3) and with CD102 (ICAM2). ICAM1 (CD54) is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase in inflammation. Also, ICAM1 (CD54) is detected in some carcinoma and melanoma cells. Soluble ICAM1 (CD54) is detectable in the plasma and is elevated in patients with various inflammatory syndromes. |
| Immunogen | Tissue / cell preparation (Human). (Burkitt?s lymphoma cell line RAJI). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | MEM-111 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIHC-PWB |
| ELISA: Use at an assay dependant concentration. The antibody reacts with soluble ICAM-1 in human serum. FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions.FACS:2 μg/ml.IHC-P:10 μg/ml. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanRatBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BB2 CD54 ICAM 1 ICAM-1 Intracellular Adhesion Molecule 1 |
|---|---|
| Target | ICAM1 |
| Tissue Specificity | This antibody reacts with human CD54 antigen. |
Application
Western blottin analysis of CD54 expression in TNF-alpha activated (A) and nonactivated (B) HUVEC cells by Lower bands represent tubulin as a loading control.| Application Notes | ELISA: Use at an assay dependant concentration. The antibody reacts with soluble ICAM-1 in human serum. FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions.FACS:2 μg/ml.IHC-P:10 μg/ml. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
