Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ID2 mouse monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ID2.Application
Immunofluorescent staining of HeLa cells using anti-ID2 mouse monoclonal antibody .Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ID2 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ID2 mouse monoclonal antibody.| Product Name | ID2 antibody [10C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [10C3] to ID2ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression. |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 1-134 of human ID2 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 10C3 |
| Host Species | Mouse |
| Tested Applications | ICC/IF,IHC,IHC-P,WB |
| IHC 1:50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC26389, ID2A, ID2H, GIG8, ID2 |
|---|---|
| Target | ID2 |
| Tissue Specificity | is specific for amino acids 1-134 of human ID2 |
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ID2 mouse monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ID2.
Application
Immunofluorescent staining of HeLa cells using anti-ID2 mouse monoclonal antibody .
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ID2 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ID2 mouse monoclonal antibody.| Application Notes | IHC 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
