Application
Wild-type (WT) and IDE knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [GT143] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
IDE antibody [GT143] detects IDE protein at cytoplasm in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. IDE antibody [GT143] diluted at 1:200.Application
IDE antibody [GT143] detects IDE protein at cytoplasm in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. IDE antibody [GT143] diluted at 1:200.Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [GT143] diluted at 1:1000.| Product Name | IDE antibody [GT143] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT143] to IDE (insulin-degrading enzyme)This gene may belong to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human IDE. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT143 |
| Host Species | Mouse |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Dog, Rhesus Monkey, BovineMouse (98%), Rat (98%), Zebrafish (90%), Dog (99%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IDE antibody , INSULYSIN antibody , insulin degrading enzyme antibody |
|---|---|
| Target | IDE |
| Molecular Weight(MW) | 118 kDa (note) (4) |
| Cellular Localization | Cytoplasm |
Application
Wild-type (WT) and IDE knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [GT143] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
IDE antibody [GT143] detects IDE protein at cytoplasm in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. IDE antibody [GT143] diluted at 1:200.
Application
IDE antibody [GT143] detects IDE protein at cytoplasm in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. IDE antibody [GT143] diluted at 1:200.
Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [GT143] diluted at 1:1000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
